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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore may well contribute to tumorigenesis. This protein was identified to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial part in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants have been reported but specific nucleotide sequence details is not still available.
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Blocking peptides are peptides that bind exclusively to the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies read more certain to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation designs which add into the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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